Journal: Journal of Inflammation Research
Article Title: Macrophage Infiltration Correlated with IFI16, EGR1 and MX1 Expression in Renal Tubular Epithelial Cells Within Lupus Nephritis-Associated Tubulointerstitial Injury via Bioinformatics Analysis
doi: 10.2147/JIR.S489087
Figure Lengend Snippet: Renal pathology and expression of IFI204, EGR1 and MX1 in tubular epithelial cells in LN. ( A ) HE staining of control mice kidney tissue sections. ( B ) HE staining of MRL/lpr mice kidney tissue sections. ( C ) The protein of IFI204, EGR1 and MX1 levels were examined in protein extracts from tubular epithelial cells of MRL/lpr or C57BL/6 mice, and ( D-F ) RT-qPCR for the levels of mRNA levels of EGR1, MX1 and IFI204 of tubular epithelial cells isolated from MRL/lpr or C57BL/6 mice. All data were shown as mean ± SD.
Article Snippet: The following primers were obtained from Metabion (Martinsried, Germany): EGR1 sense: CGTCCTGTTCCCTTTGACTT , antisense: GCATGTGATGGAGAGGATACTG ; MX1 sense: AGATGAGGAGAGAGGAGCTATG, antisense: CCAGTACATCCTCCTGACAAAG; IFI204 sense: TGACTTAGCTGCCTACCTACT, antisense: GCTGAGCCTTCCTGGATATTT; β-actin sense: GGCTGTATTCCCCTCCATCG, antisense: CCAGTTGGTAACAATGCCATGT. β-actin was used as an internal reference.
Techniques: Expressing, Staining, Control, Quantitative RT-PCR, Isolation